Porti & ambiente — le notizie raccolte

Abbiamo visto ieri, Primo Maggio, i sindacati confederali a Porto Marghera, con la tradizionale manifestazione nazionale con l’intervento dei massimi leaderdiCgil, Cisl e Uil. Margheraè un luogo cruciale dello sviluppo industriale e portuale italiano, a partire dalla sua fondazione nel 1917 e la nascita di uno smisurato polo chimico diventa anche un epicentro delconflitto tra lavoro, salute e ambientenel capitalismo contemporaneo. Un conflitto che divide la stessa comunità operaia. Anche di questo Marghera è un esempio storico importante, soprattutto tra gli anni Ottanta e il primo decennio di questo secolo, quando una frattura radicale contrappone, attorno alle sorti delpetrolchimico, lavoratori eforze sindacaliepolitiche agli ambientalistie asparute componenti operaie. Uno scontro che si conclude con un referendum comunale (a cui partecipa l’intera cittadinanza di Venezia) che boccia losviluppo del ciclo del cloro(con la conseguente chiusura di interi settori del petrolchimico). Si tentano anche vie negoziali a un modello diverso, ma si giunge solo a effimeri accordi, con aziende mai inclini a rispettarli e senza una regia politica e istituzionale (in particolare da parte dello Stato, unica entità in grado di governare una simile transizione). Così, la forza dell’ambientalismo e di un’opinione pubblica determinata a non lasciarsi più inquinare o avvelenare riesce a bloccare il vecchio modello, che peraltro perde via via capacità occupazionale, ma non è in grado di imporre una transizione autentica. Porto Marghera è oggi una zona industriale con grandi spazi bradi e inquinati,senza nessun piano di rigenerazione. L’assenza di una vera politica industriale, che affligge l’Italia da decenni, produce pure quest’abbandono di territori a lungo sfruttati e avvelenati, nella più cinica e disinvolta tradizione del capitalismo assecondata dal governo politico. La “situazione Marghera” descrive esattamente lo stato attuale del rapporto tra lavoro e ambiente e salute. O meglio, tra capitale e lavoro/ambiente/salute, poiché la vera dicotomia, il vero conflitto irriducibile, è descrivibile piuttosto in questi termini. In genere, si parla infatti più di conflitto tra lavoro, da una parte, e ambiente e salute dall’altra. Ma il lavoro vivo, il lavoro umano, è in realtà parte dell’ambiente, perché i corpi che vi sono coinvolti, la cui salute è direttamente colpita, sono elementi naturali quanto l’aria, l’acqua, la terra, e subiscono la stessa nocività. Il conflitto reale è tra ilmodo di produzione capitalistico e la natura umana, parte inestricabile della natura tutta, come insegnano l’ecologismo più consapevole e l’ecofemminismo, ma anche l’esperienza storica di luoghi come Marghera e innumerevoli altri nel mondo. Che si tratti dilavoratori esposti all’amianto o alle polveri(o anche “solo” alla fatica) o di abitanti che bevono acqua contaminata (da Pfas, ad esempio) o di un intero territorio pervaso dalle polveri sottili o cementificato o dissestato o, in molti modi, impoverito di biodiversità, si tratta sempre di un conflitto tra modo di produzione (o di consumo, di mobilità, di sviluppo urbano coerente con quello) e ambiente (che integra il lavoro umano e la vita umana e la sua salute). Il primo passo verso una riformulazione del tema, forse anche verso un nuovo soggetto capace di affrontarlo in modo organizzato, sui territori e sul piano globale, consiste proprio in questa diversa e più vasta e radicale dicotomia, in cui il lavoro sta dal lato della parte lesa e della sua in-sofferenza, dalla quale può derivare una nuova fase di conflitti. Una nuova“agency” dell’insubordinazionealla nocività nei residui luoghi di maggiore concentrazione o nei luoghi dispersi del lavoro decentrato come nelle solitudini del lavoro atomizzato. Unanocività fisico-chimica, che colpisce il corpo della terra e dei suoi elementi come il corpo umano, e unanocività psichica(da alienazione o depressione o da ecoansia) che, attraverso il nostro organismo, permea comunque il nostro ambiente e l’esperienza che ne facciamo. La sofferenza inferta è una, e tale può e dev’essere la rivolta. Non è sempre facile oggi, nella frantumazione di classe e nella parcellizzazione e nelle turbolenze delle soggettività, ma quella che si annuncia può essere la rivolta più profonda ed estesa che abbia mai attraversato il pianeta e la storia. Oggi sappiamo che la crisi ambientale esiste e che riguarda tutti noi. Se la transizione ecologica è entrata a far parte dell’agenda politica, da più parti si lamenta la lentezza con cui governi e istituzioni sovranazionali adottano misure concrete per fronteggiare l’allarme clima. La scienza, da parte sua, preannunciava già dalla metà del secolo scorso i possibili scenari futuri. Accanto a quella climatica, già nel 1972 si parlava di crisi alimentare, di inquinamento e di risorse non rinnovabili. Ma in quale preciso momento e soprattutto in che modo nel corso della seconda metà del Novecento è affiorata – nella comunità scientifica, nei movimenti d’opinione, nel dibattito pubblico – la questione ecologica? Quando ci siamo accorti che le risorse erano finite?Quando abbiamo capito – anche a fronte di disastri ambientali e catastrofi industriali che hanno prodotto traumi collettivi – che il nostro è un ecosistema fragile e che dalla sua salute dipende il benessere di tutti noi? Vai alla pagina

Stay Connected Bradley PenistonandLauren C. Williams Apex wants to provide satellite buses to primes building proliferated constellations, which means building at scale, CEO Ian Cinnamon says. The company recently announced plans to make an XL version of itsCometsatellite bus, adding power and mass yet still remaining small enough to fit 16 on a Falcon 9, Apex CEO Ian Cinnamon said in a recent interview. But, he said, “I think what everybody tends to forget about is the need to really build these at really high rate production. And that's really the focus around Comet.” Cinnamon said Apex builds at scale by reusing avionics and other systems from its earlier, medium-sized Nova bus—but also by using Octopus, the custom software that he calls his company’s “secret sauce.” “Effectively, it's not even our manufacturing operating system; it's our entire company operating system,” he said. “It controls everything from, you know, forecasting demand, understanding on inventory, receiving quality from suppliers. How many, you know, kits we're holding on inventory at any given time, all the way to work, instructions on the factory floor, tracking, NCRs, traceability on orbit.” Like seemingly everything else these days, Octopus “uses a significant amount of artificial intelligence, a significant amount of software automation for processes,” the CEO said. But what Apex doesn’t use—at least not yet—is industrial robotics. “We do not believe that automation and robots, in terms of the hardware manufacturing, makes sense at all at the scale that we're building,” he said. “It does not make sense at the scale of hundreds of vehicles per year…When you go above that scale, it may begin to make sense.” Welcome You’ve reached the Defense Business Brief, where we dig into what the Pentagon buys, who they’re buying from, and why. Send along your tips, feedback, and song recommendations tolwilliams@defenseone.com. Check out the Defense Business Brief archivehere, and tell your friends tosubscribe! A new ad is pushing Congress to fund American shipbuildingto combat China and its growing commercial and naval fleets—and also increase jobs and domestic manufacturing. TheUSA Shipbuilding Coalition, composed of shipbuilders and unions,releasedthe video Wednesday—with timing that appears to coincide withSHIPS Actlegislation challenges. Defense tech startup Firestorm Labs snags $82 millionin its Series B funding round led by Washington Harbour Partners, which said the company is “building a new model for manufacturing.” The new capital will help Firestorm increase production of itsmobile 3D-printingxCell platform that can be set up in a matter of hours, manufacturing everything from drones to prosthetics to tools. One more manufacturing thing:Pentagon-funded institutionBioMADEis putting up$21.4 millionto expand domestic biomanufacturing. The funds will support 14 projects in areas like biosensors to help detect disease, plastics used for 3D printing, and proteins for wound healing and chemical defenses. NEXT STORY:First protest filed against the Army’s troubled MAPS contract

Sinolam International, a Singapore-based investment company focused on oil, gas, and power investments in emerging markets in Asia and Latin America, has disclosed the return of its multibillion-dollar lawsuit against AES Corporation to the Virginia state court. This legal challenge, which revolves around Panama’s liquefied natural gas (LNG)-to-power market, is connected with the cancellation of a license for a major gas-fired power generation project. According to Sinolam, a U.S. Federal District Court in Virginia remanded itslawsuitagainst AES Corporation, originally filed on December 19, 2025, in the Circuit Court for Arlington County, back to the Virginia state court on April 24th, granting the firm’s request over the other player’s objections. The Panamanian company claims to have successfully sought to argue the case in Virginia, where corporate entities are held highly accountable for ethical lapses. Sinolam is seeking more than $4 billion in the U.S. case. Sinolam LNG TerminalandSinolam Smarter Energy LNG Power Co., which are energy infrastructure developers focused on LNG-to-power solutions in emerging markets,welcomedthe $33.4 billion AES acquisition by the BlackRock-led consortium, as it could strengthen financing in the context of any future resolution of the litigation. This content is available after accepting the cookies. $33.4 billion acquisition of AES filling financial coffers for Panamanian firms’ $4B lawsuit As a result, the case will now move forward in Arlington County, where Sinolam highlights that key decisions were mostly orchestrated by AES management from the company’s global headquarters there. The Panamanian player alleges AES, along with partner InterEnergy Holdings, worked to exclude it from participating in Panama’s LNG-to-power market, pointing to alleged misuse of confidential information, interference with contracts, and intimidation tactics. The company emphasizes that it had already secured permits and commercial agreements for an LNG terminal and power project, but those plans could not progress due to“the unlawful actions of AES and InterEnergy, acting by themselves and through their joint venture, Group Energy.” Kenneth Zhang, Sinolam’s CEO, commented:“We are pleased with the decision to return this matter to Virginia state court and appreciate the clarity it brings to the path forward. Sinolam remains confident in the strength of our claims and is committed to pursuing them vigorously in the appropriate forum.” Take the spotlight and anchor your brand in the heart of the offshore world! Join us for a bigger impact and amplify your presence at the core hub of the offshore energy community!

This 2012 Toyota FJ Cruiser Trail Teams Special Edition has 67k miles and has been modified for overland use with the installation of an Overland Vehicle Systems collapsible shower stall, a Glind hot shower kit, a custom-fabricated sleeping platform/galley system, a two-burner cooktop, a cargo drawer, and a Dometic refrigerator. Finished in Radiant Red over Dark Charcoal and red upholstery, the truck is powered by a 4.0-liter V6 linked with a five-speed automatic transmission, a dual-range transfer case, and a locking rear differential. It rolls on 16″ TRD alloy wheels and is further equipped with Bilstein shocks, A-TRAC traction control, LED driving lights, a backup camera, rocker panel guards, air conditioning, and a Hieha touchscreen head unit with smartphone connectivity. Acquired by the seller in 2020, this modified FJ Cruiser is now offered with manufacturer’s literature, a VIAIR compressor, a Baofeng handheld radio, an Icom HAM radio, water and gasoline jerry cans, various spare parts, a clean Carfax report, and a clean California title in the seller’s name. All Trail Teams Special Edition examples were finished in Radiant Red for the 2012 model year. An Overland Vehicle Systems collapsible shower stall has been mounted to the roof rack and is paired with a Glind hot shower kit. Additional details include LED front driving lights, illuminated mirror markers, rocker panel guards, and a receiver hitch. The bicycle rack shown in the photograph is not included in the sale. Black-finished 16″ TRD alloy wheels are wrapped in 265/75 BFGoodrich All-Terrain T/A KO2 tires with a mixture of 2020 and 2021 date codes, and a matching spare is affixed to the swing-away tailgate. The truck is equipped with TRD Bilstein shocks and Active Traction Control (A-TRAC). The cabin features front bucket seats and a 60/40-split folding rear bench trimmed in Dark Charcoal upholstery with red cloth inserts. A touchscreen Heiha head unit with Apple CarPlay and Android Auto connectivity as well as a USB power port has been installed, and further appointments include air conditioning, cruise control, a backup camera, a Trail Teams Edition dashboard emblem, and WeatherTech all-weather floor mats. The two-tone leather-wrapped steering wheel frames a 120-mph speedometer and a tachometer with a 5,500-rpm redline alongside gauges for voltage, coolant temperature, and fuel level. A dash-mounted auxiliary pod houses a compass, a digital clock, and an inclinometer. The digital odometer indicates 67k miles, approximately 26k of which have been added under current ownership. The cargo compartment has been fitted with a custom-fabricated sleeping platform/galley system, a two-burner liquid-propane cooktop, a Dometic CD-30 refrigerator, and a storage drawer. The 4.0-liter 1GR-FE V6 was factory rated at 260 horsepower and 271 lb-ft of torque. The battery was replaced in January 2024, and the vehicle most recently passed a California emissions test in March 2026. Power is routed to the rear or all four wheels through a five-speed automatic transmission, a dual-range transfer case, and a locking rear differential. The Carfax report is free of accidents or other reported damage. The winning bid does not include shipping. It is the buyer's responsibility to arrange the details of any shipping or delivery, and to pay any taxes, duties, or charges associated with shipping or delivery.View our third-party shipper recommendations. We need to confirm your billing address in order to appropriately charge fees and taxes should you win an auction. Please provide your billing address below. Congratulations! You're the high bidder. Your bid has been posted in the comment flow on the listing, and you can see other bids there as they happen. Good luck! 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Perchloroethylene, a chemical commonly used in the dry-cleaning sector, presents a potential risk to kidney health. This study measured urinary and blood nephrotoxic biomarkers among dry cleaners compared to hotel laundry workers to assess PCE’s impact. A multi-center comparative cross-sectional study was conducted with 164 randomly selected participants from 21 dry-cleaning shops and 26 hotel laundries. Data were collected through biological samples and structured questionnaires and with tests for statistical significance. The result showed that majority of the employees were females in both groups and the risk of nephrotoxicity was higher in dry cleaners as compared to hotel laundry workers. We found a significant mean difference in three biomarkers namely, Total Protein (TPU) with a Median & IQR value of (102 mg/dl &70.75 mg/dl) and (54.5 mg/dl &27.25 mg/dl), Urinary creatinine with a Median & IQR value of (193 mg/dl & 111.06 mg/dl) and (142.93 mg/dl & 78.17 mg/dl) and Urinary Calcium with a Median & IQR value of (2.60 mmol/l & 2.94 mmol/l) and (0.835 mmol/l & 0.79 mmol/l) for the exposed and the control groups respectively. However, a significant difference was not found in urinary protein to creatinine ratio, urinary sodium, Blood urea nitrogen and S. creatinine between the two groups, but higher value of sodium above range and higher BUN within range was observed in dry cleaners and factors like employment duration, PCE spillage, handling frequency, sex, and poor ventilation correlated with immediate symptoms. In conclusion, dry cleaners are at greater risk of kidney damage linked to PCE exposure, warranting implementation of safety measures and regular health monitoring to protect workers. Citation:Seid PM, Habtu W, Shentema MG, Abegaz T (2026) Risk of nephrotoxicity among dry cleaning workers exposed to perchloroethylene: A comparative cross-sectional study in Addis Ababa, Ethiopia. PLoS One 21(4): e0348427. https://doi.org/10.1371/journal.pone.0348427 Editor:Hean Teik Ong, Universiti Tunku Abdul Rahman Fakulti Perubatan dan Sains Kesihatan M Kandiah, MALAYSIA Received:February 7, 2026;Accepted:April 16, 2026;Published:April 30, 2026 Copyright:© 2026 Seid et al. This is an open access article distributed under the terms of theCreative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability:All relevant data are fully available without restriction. The minimal dataset underlying the results of this study is provided asSupporting Informationfiles. S1 and S2 Data are provided in SPSS (.sav) format, while S3 and S4 Data are the same datasets provided in comma-separated values (.csv) format to enhance accessibility and usability. All data have been fully anonymized and do not contain any identifying information. Funding:This research was funded by Addis Ababa University’s College of Health Sciences in Addis Ababa, Ethiopia, and Norwegian Program for Capacity Development in Higher Education and Research for Development (NORHED II) in Low-Income Countries research project. The funder had no involvement in the study’s design, data collection, analysis, interpretation of the findings, and manuscript writing for publication. Competing interests:The authors have declared that no competing interests exist. Perchloroethylene (PCE), also known as tetrachloroethylene, which is a colorless, non-flammable liquid solvent primarily used in the dry cleaning industry, has been known to cause significant public health problems [1]. The application of PCE as a cleaning solvent started in the 20th century [2]. It is also an important metal-degreasing solvent and an intermediate for other chemical production [3]. It is slightly soluble in water, having a boiling point of 121.4 °C, and a higher density than air, which makes good ventilation in an occupational setting mandatory [2]. Dry cleaners are the most occupationally exposed group to PCE [4]. Inhalation is the major route of entry [5] and It takes 3 days for half of it to be removed from a human body [1]. PCE has been identified by the International Agency for Research on Cancer as a probable carcinogen, with a very limited study on humans [6]. It has both acute and chronic health impacts, causing many health issues affecting the nervous system, kidneys, and liver [1]. It is believed to be a nephrotoxic, skin, and respiratory irritant [4]. During the 1990s, the use of perchloroethylene (PCE) increased rapidly, particularly in dry-cleaning shops where it became a commonly used solvent. This trend drew the attention of public health researchers, prompting efforts to assess occupational exposure. Consequently, numerous exposure assessment studies were conducted during this period [6,7]. Some health effect studies have been conducted in Western and Asian countries suggesting a link between perchloroethylene (PCE) exposure and nephrotoxicity [7–9]. Kidney toxicity, including both acute kidney injury (AKI) and chronic kidney disease (CKD), is a major global health concern, affecting an estimated 10% of the world’s population [10]. Research indicates that workers exposed to perchloroethylene (PCE), such as those in the dry-cleaning industry, are at increased risk of developing kidney-related health problems [5]. Unfortunately, consistent and conclusive evidence linking PCE exposure to kidney toxicity remains limited. The American Conference of Governmental and Industrial Hygienists (ACGIH) suggests conducting further research regarding the health effects of PCE [7]. A study conducted in Italy also highlights the need to do research on the health effects of PCE at even low occupational doses [11]. The metabolism of PCE in the human body takes two pathways [12]. The first one is oxidative metabolism, which involves P450to convert it into TCA(trichloroacetic acid) and others, causing liver and kidney toxicity [13]. The second metabolism is GSH (glutathione conjugation), which results in nephrotoxic metabolites and causes acute proximal tubal injury and kidney damage [14]. but the effect of GSH metabolites on kidney toxicity was not assured, but rather hypothesized [12]. Kidney toxicity is one of the critical concerns of PCE health effects [5]. Chronic exposure to it has been associated with end-stage renal disease [8]. Few studies have tried to study the renal effects of PCE among dry cleaners and found inconsistent results [1]. Since the practice of dry cleaning is increasing in Ethiopia, it is crucial to understand the extent of its health impacts among dry cleaners to adopt and implement safer working practices. Biomarkers are essential in understanding the nephrotoxic effects of PCE. Proteinuria serves as a key biomarker for detecting PCE-induced nephrotoxicity. Some studies have shown elevated total protein (TPU), indicating glomerular injury from PCE toxicity in both animals and humans [15,16]. However, other research has found no significant or consistent association, highlighting inconsistent evidence on this effect [17]. According to a case report in South Korea, urinary calcium levels are elevated due to calcium crystals in the renal tubules caused by PCE exposure [18]. Similar to this, low urinary sodium excretion often precedes the diagnosis of AKI, especially in critically ill patients [19]. BUN and S. creatinine, which are standard kidney function biomarkers [20], would show impaired urea excretion and reduced GFR, respectively, as a result of PCE exposure toxicity [15,16,19]. Despite acknowledging limitations in our search coverage, we found no notable studies on PCE exposure and its health effects in Africa or Ethiopia. This highlights a significant knowledge gap, especially as the dry-cleaning sector continues to grow in the region. In Ethiopia, the increasing number of dry-cleaning shops has raised concerns about occupational exposure to perchloroethylene (PCE), making it an emerging public health issue. This study investigated the relationship between perchloroethylene (PCE) exposure and nephrotoxicity in Ethiopia, where no prior data on this association currently exists, and tried to fill the existing knowledge gap and provide evidence-based recommendations for improving occupational health standards in the Ethiopian dry-cleaning industry. A multicenter comparative cross-sectional study was conducted in Addis Ababa from March 8, 2025, to April 7, 2025. Using simple random sampling from the “addisbiz” local directory, 17 of 62 dry-cleaning shops were chosen by lottery, enrolling 70 dry cleaners. Similarly, 21 of 547 hotels were randomly selected to recruit 70 non-exposed controls. For blood sampling, an additional 4 dry-cleaning shops and 5 hotels (from unselected establishments) were randomly picked, with 12 participants obtained from each group. Hospitality laundry workers were selected as the control group because they perform physically and environmentally comparable tasks such as handling, washing, ironing, and processing textiles in similar hot, humid workplace conditions with exposure to water-based detergents and mechanical stressors, yet without routine use of perchloroethylene solvents. We have selected only those hotels that doesn’t provide dry-cleaning service. This design helps isolate the potential nephrotoxic effects of PCE while minimizing confounding from shared socioeconomic factors, occupational physical demands, shift patterns, and general service-sector exposures common in low- to middle-income settings like Addis Ababa. Alternative control populations, such as office workers or the general community, were considered but deemed less suitable due to substantial differences in physical workload, heat/humidity exposure, and socioeconomic status, which could introduce bias in biomarker comparisons related to kidney function. Similar control selection strategies (e.g., using non-solvent-exposed laundry workers) have been employed in prior occupational studies of PCE-exposed dry cleaners to enhance comparability and internal validity. The sample size was calculated using the formula for comparative cross-sectional studies. However, because we could not identify definitive values for the effect size, mean, or standard deviation for both groups and the available data only pertained to workers’ exposure status, which would have greatly limited our sample size for assessing nephrotoxicity, we opted to use Cohen’s d. We assumed a medium effect size of 0.5, which is commonly considered appropriate for detecting meaningful differences in public health studies with a 1:1 allocation ratio Cohen’s d is calculated as: Assuming equal variances between the two groups, the sample size calculation was simplified by using a common standard deviation (SD). To account for an anticipated 10% dropout rate, the initial sample size (n = 63 per group) was adjusted using the formula: New sample size = n/ (1 − dropout rate). This resulted in a required sample size of 70 participants per group (63/ 0.9 ≈ 70), yielding a total of 140 participants included in the study. Taking the mean and standard deviation values for serum creatinine from a health impact assessment conducted in Iran [21], the sample size was calculated using the following formula. Based on the computation, the initial sample size was 10 per group. After accounting for a 10% dropout rate, the final sample size was adjusted to 12 participants per group. Assuming a one-to-one allocation ratio, a total of 24 participants were included, comprising 12 dry cleaners and 12 hotel laundry workers. Workers aged 18 and older, employed for at least six months in dry-cleaning or hospitality laundry services, and able to give informed consent were eligible for the exposed group. Exclusions included those with pre-existing kidney disease before employment, those on temporary medication for non-kidney illnesses, pregnant or lactating women, and women menstruating during data collection due to metabolite variation. Data were collected using structured questionnaires with both open-ended and closed-ended questions and biological samples. Ethical approval was obtained from Addis Ababa University School of Public Health, IRB committee with reference number SPH/452/2025, and Addis Ababa Health Bureau Ethical clearance committee with reference number 2/080/17. The study was conducted in accordance with the ethical principles for medical research involving human subjects outlined in the Declaration of Helsinki. Participants were asked to sign informed consent before collecting urine and blood samples, and were briefed to leave if they wanted to at any part of the research process without any constrictions. After getting their consent, willing participants were guided by trained collectors to provide clean catch mid-stream spot urine samples during their working hours following Ethiopian Public Health Institute (EPHI) protocols to reduce contamination. Urine samples, labeled with identification codes, were analyzed for nephrotoxicity biomarkers: proteinuria, creatinine, calcium, and sodium. Blood samples from 24 participants were collected by trained health professionals on Wednesdays and Thursdays after shifts to measure BUN and serum creatinine, reflecting PCE exposure. Samples were kept at 2–8°C, transported in cold boxes, and stored in EPHI laboratory refrigerators for later analysis using automated urine biochemistry analyzers. The laboratory analysis was performed at the ISO 15189:2012-accredited EPHI National Clinical Chemistry referral and Reference Laboratory. Urinary biomarkers for kidney function were quantified in all 140 participants, and serum biomarkers in 24 participants. Protein in urine/CSF was measured via a turbidimetric method using cobas analyzers (range: 40–2000 mg/L). Calcium levels were determined photometrically with cobas analyzers (serum range: 0.20–5.0 mmol/L; urine: 0.20–7.5 mmol/L). Sodium concentrations were measured by ion-selective electrode on cobas c 111 (range: 20–250 mmol/L). Urine/CSF samples for protein were centrifuged to avoid precipitates; calcium and sodium assays required separation from cells within 4 hours, avoiding silicone-gel tubes to prevent interference. Interferences such as hemolysis, icterus, lipemia, and certain drugs were monitored and mitigated according to defined thresholds. BUN was measured enzymatically (urease/GLDH method) on cobas c 501/502 (2–100 mg/dL range), and serum creatinine was determined by kinetic Jaffé methods on cobas c 503/703, calibrated to IDMS standards (0.2–25 mg/dL). Serum samples were processed within 2 hours, stored at 2–8°C. Data were cross-checked and cleaned by the principal investigator before analysis. The basic demographic characteristics of both the exposed and control group workers from the selected 17 and 21 dry cleaning establishments and hotels, respectively are shown inTable 1. The majority of workers in both laundries were females, covering 61.4% of study participants in dry cleaning and 65.7% in hotel laundry. Participants’ sex, job role, working days, hours, and years of employment are shown inTable 1along with their behavioral characteristics of smoking and drinking. https://doi.org/10.1371/journal.pone.0348427.t001 Comparison of key urinary biomarkers between dry cleaning workers (exposed group) and hospitality laundry workers (control group) is shown inTable 2. The data are expressed as median values with interquartile ranges (IQR) to illustrate the central tendency and variability within each group. This comparison highlights differences in total protein, creatinine, calcium, and sodium levels, providing insight into potential occupational exposure effects on renal function. https://doi.org/10.1371/journal.pone.0348427.t002 Blood urea nitrogen (BUN) and serum creatinine levels between an exposed group and a control group, presenting means and standard deviations (SD) is presented inTable 3. The exposed group exhibits higher mean values for both biomarkers compared to the control group, with larger SDs indicating greater variability in the data. From the total of 12 samples in each group the prevalence of abnormal BUN was 33.3% (4 workers) and 16.7% (2 workers) in dry cleaners and hotel laundry workers respectively, while 3 workers in each group showed abnormal serum creatinine level with a prevalence of 25%. https://doi.org/10.1371/journal.pone.0348427.t003 The dry cleaning group reports higher percentages of decreased urine output, foamy/discolored urine, and dizziness/headache, while the hotel laundry group shows a higher prevalence of no changes in urination and no training on PCE handling. Fatigue/weakness and nausea/loss of appetite frequencies are relatively similar between groups, with slight variations in specific symptom prevalence. Health symptoms and training experiences between the two groups is presented below inTable 4. https://doi.org/10.1371/journal.pone.0348427.t004 An independent t-test was performed to see if there was a significant, meaningful difference in urinary and serum biomarkers distribution between the exposed and the control groups inTable 5. The result showed that Log10transformed total protein, urinary creatinine and calcium had a significant association with p < 0.05 for all three. https://doi.org/10.1371/journal.pone.0348427.t005 Non- parametric Mann-Whitney U test was also conducted to see if there was a significant meaningful urinary sodium distribution difference between the exposed and the control groups, and the result showed a non-significant distribution with a p value of 0.176, which makes the null hypothesis true. The renal function of participants was evaluated by comparing the estimated Glomerular Filtration Rate (eGFR) across the two occupational groups. To ensure a standardized assessment, the eGFR was calculated for each participant using the 2021 CKD-EPI creatinine equation (race-neutral), which incorporates serum creatinine, age, and sex as variable. The Mann-Whitney U test was employed to determine if significant differences existed between the groups. This non-parametric approach compares the distribution of ranks by providing a robust evaluation of potential impairment associated with occupational chemical exposure as shown inTable 6. https://doi.org/10.1371/journal.pone.0348427.t006 A correlation analysis was performed to see which risk factors significantly influence each specific biomarker. Point-Biserial correlation was conducted between risk factors and log-transformed urinary biomarkers of kidney function, and the result showed the strength and direction of the significantly associated results as shown inTable 7. https://doi.org/10.1371/journal.pone.0348427.t007 Spearman correlation analysis was conducted as shown inTable 8, for urinary sodium and for the three urinary biomarkers to see if there is an association with variables that have an ordinal nature. https://doi.org/10.1371/journal.pone.0348427.t008 Total urinary protein less than 150 mg/dl is considered normal according to the EPHI guiding reference. From a total of 140 samples, 17 results showed deviation from the normal range, where most of the deviations are found in the dry-cleaning sector. 26 samples showed abnormal urinary creatinine results, where 29–226 mg/dl for females and 40–275 mg/dl for males were considered as a normal range. 97 tests showed abnormal urinary calcium results, where 2.5–7.5 mmol/l is considered normal. From the urinary sodium test, only 8 samples showed abnormal range, where 27–287 mmol/l for females and 40–220 mmol/l for males were considered in the normal range. The full comparison is shown inFig 1below. https://doi.org/10.1371/journal.pone.0348427.g001 A multiple linear regression analysis was conducted as shown inTable 9to examine the effects of demographic characteristics, occupational factors, lifestyle characteristics, medical history, and workplace safety practices on urinary protein-to-creatinine ratio (UPCR). The model included age, days worked, type of ventilation, water intake, family history of kidney disease, personal history of kidney disease, chemical spillage, working hours, smoking, workplace, personal protective equipment use, hand washing practice, educational status, alcohol use, and health conditions like hypertension and diabetes. https://doi.org/10.1371/journal.pone.0348427.t009 The overall model was statistically significant (F (15,124) = 2.125, p = 0.013), explaining 20.4% of the variance in UPCR (R² = 0.204; adjusted R² = 0.108). A histogram of the residuals demonstrated an approximately normal distribution and the residual-versus-fitted plot showed a random scatter of points, indicating that the assumptions of normality and homoscedasticity were met. In addition, all predictors had tolerance values greater than 0.1 and variance inflation factor (VIF) values below 10, indicating absence of multicollinearity. Increasing age was significantly associated with lower UPCR (B = −0.018, 95% CI: −0.029, −0.007; p = 0.002). Workplace category was positively associated with UPCR, with participants in the exposed workplace having significantly higher UPCR compared with the reference group (B = 0.209, 95% CI: 0.059, 0.359; p = 0.007). Use of personal protective equipment was significantly associated with lower UPCR (B = −0.175, 95% CI: −0.300, −0.050; p = 0.006). Alcohol use was also significantly associated with higher UPCR (B = 0.096, 95% CI: 0.003, 0.190; p = 0.044). Other variables including days worked, type of ventilation, water intake, family history of kidney disease, personal history of kidney disease, chemical spillage, working hours, smoking status, hand washing practice, educational status, and general health condition were not significantly associated with UPCR in the adjusted model (p > 0.05). A multiple linear regression analysis was also conducted inTable 10below to examine the effects of PCE exposure predictors, demographic variables, health-related factors, and workplace safety practices on urinary total protein (mg/d). The model was statistically significant (F(14, 125) = 4.156, p < 0.001), and explaining 31.3% of the variance in proteinuria (R2= 0.318 and adjusted R2= 0.241). A histogram plot of the residuals showed a bell-shaped distribution, a residual verses fitted value plot showed a random scatter of points, all predictors Variance Inflation Factor (VIF) was below 10 and tolerance value for all factors were above 0.1, by which normality, homoscedasticity and absence of multi collinearity assumptions were met for the model. https://doi.org/10.1371/journal.pone.0348427.t010 A multiple linear regression was also conducted to see the effect of predictors to urinary creatinine level (mg/dl) after checking the assumptions in the same fashion done above for TPU. 10 predictors were included in the model, age, sex, BMI (Body Mass Index), work place, year of employment, working hour, water intake, smoking, alcohol consumption, history of kidney disease. The model was statistically significant (F(10, 129) = 2.412, p < 0.012), and explained 15.7% of the variance in urinary creatinine (R2= 0.157 and adjusted R2= 0.092). Only two predictors were significant. The first one is a place of work, which had a significant positive effect (β = 0.152, p = 0.009), indicating that being a dry-cleaning worker is associated with 1.42 times increase in urinary creatinine, holding other variables constant. And the second one is years of employment (β = 0.051, p = 0.038), indicating that a one-year increase in laundry establishment results in 1.12 times increase in urinary creatinine, holding other variables constant. Separate regression analysis was done for this two urinary biomarkers by adding approximated categorical dietary intake of sodium and calcium predictors from the recent meal they had before sample collection along with predictors like age, sex, BMI, place of work, working hour, water intake, smoking status, alcohol consumption, past history of kidney disease, experience of PCE spillage and availability of adequate ventilation. the model for urinary calcium was statistically significant (F(14, 124) = 4.21, p < 0.001), and explaining 32.9% of the variance in urinary calcium (R2= 0.322 and adjusted R2= 0.246), however the only significant predictor was place of work which had a significant positive effect (β = 0.438, p < 0.001), indicating being a dry cleaning worker is associated with a 2.74 times increase in urinary calcium, holding other variables constant. Regression analysis for urinary sodium showed a non-significant result in both the model and in all of the predictors, as a result, we excluded it from the regression report. From the four urinary and two serum biomarkers, TPU was the most crucial and directly linked to Perchloroethylene induced nephrotoxicity [22]. Different studies have identified urinary protein levels as a sensitive indicator of kidney damage caused by toxic substances [23,24]. Our result showed a significant increase in proteinuria in dry cleaners as compared to hotel laundry workers, with a P-value of 0.001 in both parametric and non-parametric tests. Similar to our findings, studies conducted in Italy, Germany and the Netherlands to assess the renal effects of PCE in dry cleaners showed a significant increase in proteinuria in the exposed groups compared to the control groups [15,24,25]. Dry cleaners accounted for 88.2% of observed abnormal TPU (>150mg/d) in our study, while 87% of subjects in the Italy study showed an increased high molecular weight protein in urine, which was associated with tubular alteration [25]. On the other hand, some studies contradicted our finding. A study conducted in the US found no direct association between PCE exposure and urinary biomarkers from 192 dry cleaning workers, even though protein/creatinine showed a weak positive association [17]. In contrast to the previous studies, a study conducted in the Czech Republic found no significant difference in total urinary protein between the exposed (125 mg/g) and control group (103 mg/g), countering the general claim of a clear association between PCE exposure and proteinuria [16], Prevalence of abnormal value of protein showed no significance between the exposed and control groups in another study conducted in the Netherlands [26]. Whereas abnormal TPU in our findings showed a significant association by which hotel laundry workers had 88.2% lower odds of TPU abnormality compared to dry cleaners. According to the agreed-upon pathophysiological study of Proteinuria, PCE exposure results in higher protein in urine by compromising the glomerulus, which is the key filtration unit within in nephron that allows the passage of tiny water and electrolyte molecules, while blocking large molecules like protein [27]. When this filtration unit is compromised by PCE (controlling for other factors like diabetes, hypertension), protein output in urine increases [12,28]. Urinary creatinine was used to show the effect of PCE on kidney filtration as a result of toxic-induced renal damage, resulting in GFR reduction [29]. It is a key indicator of overall kidney function, since it is a result of creatine degradation from muscle [30]. However, most of the time, it is used as a normalizer for other biomarkers [31]. PCE exposure is believed to decrease GFR [29], which in turn decreases urinary creatinine and indicates acute and chronic kidney disease [32]. Some studies used S. creatinine only as a biomarker to detect nephrotoxicity, but found no significant association, which is similar to our S. creatinine finding. The results of a study in Iran found no significant difference in Cr levels between dry-cleaners (1.1 mg/dl) and control group (0.92 mg/dl) [21]. Similarly, a study conducted in China to see the effects of the exposure on liver and kidney functions yielded negative results as judged by the emission enzyme activities [33]. Complimentarily a study in Italy found that chronic exposure to organic solvents did not significantly alter urinary creatinine level, although it stated that urinary creatinine can’t be used to detect renal dysfunction alone [34]. Our research found a significant urinary creatinine difference between the exposed and the control group, however majority of them showed an increased urinary creatinine above the range (>226 mg/dl for female and >275 mg/dl for males). Which might come from renal tubular damage caused by PCE metabolites like S-(1, 2, 2-trichlorovinyl)-L-cysteine (TCVC), which induce oxidative stress and affect tubular integrity [15,30]. As a result kidneys’ ability to filter will decrease, and GFR would fall, so to compensate for this, the proximal tubules would increase creatinine secretion and another possibility is tubular epithelial barrier might be compromised by PCE metabolites, resulting in passive leakage of Cr to urine [35]. From this, we can hypothesize that the non-significant result of creatinine from the above research could be due to the compensation secretion of creatinine by proximal tubules or due to leakage of Cr through compromised tubular epithelial barrier during the early stages of PCE Impact on kidney. The urinary protein-to-creatinine ratio (UPCR) is a validated, spot-urine index that normalizes protein excretion to creatinine as a surrogate for glomerular filtration rate and urine concentration [36]. In clinical practice, spot urinary protein measurements are highly influenced by hydration status and urine volume, whereas creatinine is excreted at a relatively constant rate proportional to muscle mass, making it a reliable indicator of urine dilution [37]. In this study, both urinary protein and creatinine levels were significantly elevated in the dry cleaning group (P < 0.001 and P < 0.002, respectively); however, when expressed as a ratio, the effect of urine concentration is mathematically adjusted, resulting in a non-significant difference in UPCR (P = 0.791). This suggests that the higher absolute protein levels observed among dry cleaning workers likely reflect more concentrated urine rather than a true increase in renal protein excretion compared to hospitality workers. Similar with previous studies, absolute urinary protein levels may vary due to transient factors such as hydration and physical activity, whereas the UPCR provides a more stable and reliable indicator of renal function by accounting for urine concentration variability and enhancing diagnostic specificity by minimizing the confounding effects of variations in creatinine excretion [37,38]. Based on a few PCE-induced tubular damage studies, we formulated a scientifically relevant hypothesis related to urinary calcium and sodium and tested both of them. Urinary calcium is an important indicator of tubular function, which could detect early kidney damage caused by PCE, since the GSH metabolite results are believed to cause tubular toxicity [22]. Kidney reabsorbs calcium in order to balance body’s Ca level. But if anything happens to the kidney, such as being compromised by PCE exposure, hypercalcemia could be seen in urine [39]. As calcium output increases in urine, the chance of crystallization with other substances increases, leading to kidney stone or calcium phosphate formation [40]. Reabsorption of urinary calcium occurs in the proximal tubule, which would indicate a secondary change in ion handling as a result of PCE-induced toxicity, since GSH metabolites causes’ tubular damage. Urinary sodium would also show PCE toxicity in the same fashion as calcium, in addition to its ability to indicate electrolyte imbalance caused by the solvent [40–43]. Our research findings showed a median value of 2.6 mmol/l and 0.835 mmol/l for exposed and control groups, respectively. Just like our hypothesis, a higher value of calcium in urine output was seen in dry cleaners. The difference between the two groups was significant with p-value <0.001 for both independent t-test and Mann-Whitney test; however, dry cleaners account only 38.1% of the observed abnormal urinary calcium. Most of the abnormal results were below the normal range, which is less than 2.5 mmol/l. Most of the study participants have a calcium level below the normal range, but those who work in the dry cleaners have more urinary release as compared to hotel laundry workers. Urinary sodium is an important biomarker to regulate the kidneys’ ability to maintain electrolyte balance. In normal conditions, 99% of Na is reabsorbed, where 70% of it is reabsorbed by the proximal tubule, 25% by the Loop of Henle in the thick ascending limb, and the rest 5% is absorbed by the distal tubule and collecting duct [44]. PCE metabolites could impair the thick ascending limb, which is a site for sodium reabsorption. This, in turn, results in disruption in the sodium-potassium-2-chloride (NKCC2) cotransporter, which results in increased urinary sodium [29], our study didn’t find any significant difference between the exposed and control groups. Although no significant difference in BUN was found between the exposed and control groups, the data showed higher values in dry cleaners, though still within the normal range. A similar result was observed in a study conducted in Iran, where dry cleaners also exhibited higher BUN values within the normal range (17 mg/dl) than control groups (15 mg/dl) [21]. Most of the results are normal in our finding, but the higher value in dry cleaners indicates early renal stress caused by PCE exposure as a result early detection and routine monitoring would be valuable in order to prevent kidney toxicity, since long employment, PPE utilization and other factors could influence it even if we haven’t found significant correlation in our study for BUN. The study didn’t measure and quantify specific exposure assessment due to unavailability of direct reading devices and high cost of biological assessment, as a result, dose response linkage was not performed and since we used cross-sectional study, cause and effect relationship couldn’t be assured, by which the observed biomarker difference is unclear whether it was the result of cumulative exposure or recent exposure. Enzyme biomarkers such as like neutrophil gelatinase-associated lipocalin (NGAL) or kidney injury molecule-1 (KIM-1) were not assessed, due to substantially higher assay costs (often 5–10 times greater), limited commercial availability and specialized reagents/kits and we didn’t have the analytical machines for them in EPHI laboratory. While PCE is the most likely causative agent based on exposure patterns, job tasks, and literature, residual confounding from co-exposures in dry cleaning cannot be fully ruled out without targeted environmental or biological monitoring of multiple solvents. We recommend that future studies in similar settings include multi-chemical exposure assessment (e.g., air sampling or urinary metabolites of other solvents) to further disentangle contributions. The finding showed a significant difference between dry cleaners and hotel laundry workers in TPU, urinary creatinine and calcium, which suggests PCE role on those biomarkers. Although the study failed to demonstrate a significant difference in UPCR, urinary sodium, BUN and serum creatinine, a higher prevalence of abnormality was seen in dry cleaners, which aligns with the biological plausibility of PCE-induced toxicity in dry cleaners. The cross-sectional nature of the study and the absence of direct exposure assessment hindered us from concluding definitively, but still the biomarkers showed a possible physiologic change and risk of nephrotoxicity as a result of the solvent., so our findings suggested that dry cleaners need to be more cautious as they are more susceptible to kidney toxicity however, a longitudinal study is needed to confirm the cause-and-effect relation. Urinary biomarker data collected using ODK and exported to SPSS format. https://doi.org/10.1371/journal.pone.0348427.s001 (SAV) Serum biomarker data collected using ODK and exported to SPSS format. https://doi.org/10.1371/journal.pone.0348427.s002 (SAV) Urinary biomarker data collected using ODK and exported in comma-separated values (.csv) format; this file contains the same data asS1 Data. https://doi.org/10.1371/journal.pone.0348427.s003 (CSV) Serum biomarker data collected using ODK and exported in comma-separated values (.csv) format; this file contains the same data asS2 Data. https://doi.org/10.1371/journal.pone.0348427.s004 (CSV) First and foremost, We would like to express our deepest gratitude to God for giving us the courage and persistence to complete this project, next we would like to take a moment and give a Special thanks to Mrs. Fikirte Aboye for her unlimited support in every step of the research process, starting from moral support, up to involving in different material and laboratory equipment purchase. Another heart felt gratitude to Bethlehem Fiseha for her support.